New permeable cryoprotectant-free vitrification method for native human sperm
نویسندگان
چکیده
منابع مشابه
Large volume cryoprotectant-free vitrification: an alternative to conventional cryopreservation for human spermatozoa.
Vitrification is a simple and cost-effective method for the storage of human spermatozoa without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen. As a result, solidification of living cells without the formation of ice crystals is achieved during cooling. This study aimed to compare cryoprotectant-free vitrification to conventional cryopre...
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Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...
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The aim of this study was to develop and to test the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for intracytoplasmic sperm injection [ICSI] or in vitro fertilization [IVF]). To test the effect of vitrification on basic sperm parameters, each of 68 swim-up-prepared ejaculates from oligo-astheno-terato-zoospermic patien...
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Introduction Automated methods for sperm characterization in microscopic videos have some limitations such as: low contrast of the video frames and possibility of neighboring sperms to touch each other. In this paper a new method is introduced for detection of sperms in microscopic videos. Materials and Methods In this work, first microscopic videos are captured from specimens of human semen. S...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2017
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/dex281